To obtain sequence information on the Folsomia candida (Collembola) transcriptome, an EST sequencing project was conducted. Approximately 9.000 EST sequences were generated. The sequences were grouped into clusters that represent putative genes and were stored in a web-accessible database (www.collembase.org). It appears that Collembase holds a wealthy source of information applicable to many fields of science (e.g. ecology, ecotoxicology, molecular evolution and phylogenetics). The sequences in the database were used in a molecular phylogenetic study to gain additional insight into the position of Collembola within the ecdysozoa. Software packages were applied for accurate protein predictions from the ESTs. Translated sequences were blasted against all ecdysozoa sequences in the protein database of Genbank and phylogenetic trees were constructed for those sequences showing significant similarity with at least one insect species, one crustacean species and one outgroup species (C. elegans). Results of this analysis, as well as Collembase itself, will be presented.