Sunday, December 12, 2010: 1:53 PM
Sunrise (Town and Country Hotel and Convention Center)
Cytochrome P450s are involved in detoxifying insecticides and plant toxins and biosynthesizing endogenous compounds. Electrons needed for these processes are transferred from NADPH to cytochrome P450 by a cytochrome P450 partner enzyme, NADPH-cytochrome P450 reductase (CPR). Using PCR-strategy, we cloned the full length of CPR cDNA from the bed bug, Cimex lectularius (Hemiptera: Cimicidae). Double strand RNA (dsRNA) mediated knock-down in the expression of CPR significantly enhanced the sensitivity to deltamethrin in a deltamethrin resistant population from the Cincinnati area (CIN-1), suggesting P450-mediated metabolic detoxification is one of mechanisms responsible for the resistance observed in this population. The developmental and tissue specific expression of the bed bug CPR was also examined.
doi: 10.1603/ICE.2016.52480
See more of: Ten-Minute Papers, IPMIS: Physiology and Reproduction
See more of: Ten Minute Paper (TMP) Oral
See more of: Ten Minute Paper (TMP) Oral