D0021 Distinct water balance profiles for shallow diapausing, deep diapausing, and nondiapausing apple maggot pupae (Rhagoletis pomonella)

Monday, December 14, 2009
Hall D, First Floor (Convention Center)
Zachary P. Phillips , Department of Entomology, The Ohio State University, Columbus, OH
Kevin R. Patrick , Department of Entomology, The Ohio State University, Columbus, OH
Joshua B. Benoit , Biological Sciences, University of Cincinnati, Cincinnati, OH
Giancarlo Lopez-Martinez , Department of Entomology and Nematology, University of Florida, Gainesville, FL
David L. Denlinger , Department of Entomology, Ohio State University, Columbus, OH
The apple maggot, Rhagoletis pomonella, has three different pupal developmental programs: nondiapause, shallow diapause, and deep diapause. In this study, we examined the water balance requirements of nondiapausing, shallow diapausing, and deep diapausing pupae. Differences in size (dry and water mass) and percent water content were minimal between the different groups. The three developmental profiles produced distinct ranges of water loss rates; the nondiapause pupae were the most porous (0.8-1.2%/d), deep diapause pupae retaining water most effectively (0.1-0.3%/h), and shallow diapause pupae lost water at an intermediate rate (0.4-0.65%/h). The critical transition temperature was higher in diapausing than nondiapausing pupae, a consequence of additional hydrocarbons deposited on the puparium of diapausing pupae, but we have not yet determined whether there is a difference between the puparia of shallow and deep diapausing individuals. Our results identified distinct pupal water balance profiles for nondiapausing and diapausing apple maggots.

doi: 10.1603/ICE.2016.43873