D0359 Programmed cell death in intestinal cells of Bombyx mori during metamorphosis: Morphological observation of autophagy and apoptosis and expression of the ecdysone-regulated genes

Wednesday, November 19, 2008
Exhibit Hall 3, First Floor (Reno-Sparks Convention Center)
Qing-Rong Li , South China Agricultural University, Guangzhou, China
Yan-Xia Shi , South China Agricultural University, Guangzhou, China
Xiao-Juan Deng , South China Agricultural University, Guangzhou, China
Wan-Ying Yang , South China Agricultural University, Guangzhou, China
Wen-nian Zeng , South China Agricultural University, Guangzhou, China
Ya-dong Huang , Jinan University, Guangzhou, China
Qili Feng , School of Life Sciences, South China Normal University, Guangzhou, China
Yang Cao , South China Agricultural University, Guangzhou, China
During metamorphosis in insect, degeneration and dissolution of the larval tissues, which is triggered by ecdysone hormone (EH), require programmed cell death, such as autophagy and apoptosis. Morphological observation on autophagy and apoptosis and analysis of ecdysone-regulated gene expression in the epithelium cells of Bombyx mori gut indicated that the Cvt, autophagy, mitophagy and reticulophagy pathways of autophagy occurred in intestinal epithelium cells during the larval to pupal metamorphosis. The typical DNA ladder of apoptosis was also detected in the degenerating epithelium cells. The expression peaks of transcripts of the EH receptor gene BmEcR and earlier transcriptional genes BmHR39、BmE74A appeared before pupation, but BmFTZ-F 1 expressed later. All the data together suggested that the early expression of BmEcR、BmHR39、BmE74A might initiate autophagy and apoptosis, and autophagy pathway occurred dominantly and earlier than apoptosis pathway. This work was supported by the “863 project” (2006AA10A119), the “973 Project) (2005CB121000), NNSF (30370716, 30570938) of China.

doi: 10.1603/ICE.2016.39290