Water agitation: A stimulus for egg hatching of Anopheles gambiae

Females of Anopheles gambiae were blood fed for oviposition to determine the effect of water agitation on hatching of the eggs. One egg was placed into each well of a multiwell plate. The treatments were daily shaken for 4 minute. The number of hatched eggs in each multiwell plate was counted daily and recorded after and before shaking. The results showed that 72.2% eggs hatched after agitation which was significantly greater than 11.7% hatched eggs in control (α=5%). It was concluded that agitation may stimulate the hatching of An. gambiae eggs.