A cDNA library was construction by a PCR amplification method (SMART library construction method, Clonetech). The library was normalized to enhance gene discovery rate and the identification of rare transcripts. Amplified cDNA was normalized using duplex-specific nuclease method prior to library cloning. A total of 960 randomly selected clones were sequenced for both directions (average insert size of 1.2 Kb). The sequences were pre-joined for each direction of the sequences for the clone. Assembly of these sequences revealed 767 UniEST with 59 contigs. Putative functions were assigned to 400 uniESTs that exhibited strong similarity to genes in public databases. About 32% of annotated sequences are involved in protein binding activity. At least 15 putative genes are related to chemosensory, locomotion and behavioral interactions. We also found at least 100 UniEST sequences that have respective putative orthology with the genes found in insects (Drosophila melanogaster, Apis mellifera, and Tribolium castaneum) and in Caenorhabditis elegans, which allow details of analysis of the molecular evolution.
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