The varroa mite, Varroa destructor, is the most severe threat to beekeeping worldwide. Oxalic acid (OA) is widely used in Europe and Canada for varroa control in honey bee colonies. Its registration is pending in the U.S. OA is greater than 90% effective in broodless colonies and poses little risk of contaminating hive products. Although treatment recommendations have been established for colonies, techniques for treating package bees have not been investigated. The goal of my research was to develop a protocol for using OA to eliminate mites from package bees. I made 102 packages of varroa-infested adult bees. Each package contained approximately 1500 bees and represented an experimental unit. I prepared a 2.8% solution of OA by mixing 35 g OA with 1 L of sugar water (1:1 w:w). I assigned 8 treatments to the packages based on previous laboratory bioassays which characterized the acute contact toxicity of OA to mites and bees. I administered the treatments by spraying the OA solution directly on the bees through the mesh screen cage using a pressurized air brush. I quantified mite and bee mortality and estimated the optimum dosage of OA to apply to package bees for mite control without injuring bees. The outcome of my research will provide beekeepers and package bee shippers guidance for using OA to eliminate mites from package bees. Further, my results will contribute to the registration of OA and will guide beekeepers in how to use the product effectively when registered in the U.S.