The mission of the National Plant Diagnotic Network (NPDN) is to rapidly and accurately detect and diagnose introduced pests and pathogens through established protocols. Accurate and rapid diagnosis of new important pathogens such as soybean rust (Phakopsora pachyrhizi) or brown rot/southern wilt (Ralstonia solanacearum) depends on the availability of standardized protocols based on common sense and replicated research. Given the rapid rate of new introductions, distribution of expertise and training must be consistent and equally rapid. However, replication of diagnostic tests must be carried out by multiple institutions using various platforms, primers, techniques, etc. to ensure that the tests are valid and utlizing the best science available. The existence of multiple species or races complicates diagnostics, which in turn extends the time for sample turnaround and can even result in incorrect diagnoses. Vetting diagnostic techniques with several different laboratories may help to increase the robustness of current and future diagnostic protocols, aiding all laboratories in early detection.