Biological and reproductive performance of Oebalus insularis (Heteroptera: Pentatomidae), reared in artificial diets: Subsidy for the multiplication system of Telenomus podisi (Hymenoptera: Platygastridae)

Tuesday, November 17, 2015
Exhibit Hall BC (Convention Center)
Bruno Zachrisson , Entomology, Lab., IDIAP, Panama, Panama
The optimization of the system of multiplication of Telenomus podisi, depends on obtaining eggs of Oebalus insularis. So, assessed their performance biological and reproductive, reared in two artificial meridicas diets. Nymphs of the second instarof O. insularis, obtained under controlled abiotic conditions (28 oC of temperature), 80 % of Relative Humidity and 12 hours Phototophase), were fed with two artificial diets. The diet ¨A¨, prepared with flour, white rice (spray) (10 g), sucrose (2.5 g), dextrose (7.5 g), distilled (100 ml) water, wheat germ (10 g), vitamin solution (10 mg / 100 ml), nipangin (1 g) and tetracycline (25 g). Diet ¨B¨, in addition to these components,  were added 10 g Kenoa ® (E. colona, E. crus-galli). The biological and reproductive performance of O. insularis, in both artificial diets, compared with two control treatments (O. sativa, E. colona), considered natural diets. The biological and reproductive, parameters evaluated artificial diets were evaluated. The experimental design was complete random and the number of replications per treatment was of 50 individuals, whereas a nymph by ¨Petri¨ dish. Data were analyzed through ANOVA to 5% probability and subsequently the Tukey test was applied. The ¨B¨ diet, significantly reduced the total length of the cycle and increased longevity and the rate of the female O. insularis; also favoring reproductive parameters as it is the period of oviposition, number of eggs masses and number of eggs per female per masses and the viability of the eggs. In this way, the best biological and reproductive performance of O. insularis, was the diet ¨B¨.
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