Profile comparison of glycoprotein's present in the middle and posterior intestine of Meccus pallidipennis and Triatoma barberi

The process of differentiation of Trypanosoma cruzi in the intestine triatomine involves interactions can be influenced by the morphology of the insect gut or by molecules such as proteins and glycoproteins; and it has been observed that the virulence of isolates are related to vector species. Therefore the aim of this study was to compare the morphology and profile of proteins and glycoproteins middle and posterior intestine of male and female of Meccus pallidipennis and Triatoma barberi. Histological analysis of stained with PAS intestines was performed by light microscopy; and the electrophoretic pattern of SDS-PAGE and lectin affinity for recognition by Western blot residue N-acetyl-D-glucosamine, N-acetyl-D-galactosamine and a -2,3-sialic acid was observed. Morphological differences between middle and posterior intestine were observed in all experimental conditions, most important being the presence of intracellular PAS positive granules and secretion in the gut epithelium of female Triatoma barberi fasting; components 61 and 64 kDa were identified in the electrophoretic pattern with residues of N-acetyl-D-glucosamine, which in previous studies have been associated with the digestion of the insect. Alternatively, components 46 and 45 kDa were identified by residues of N-acetyl-D-galactosamine, which was previously shown to be involved in the process-intestinal parasite recognition vector. The observed differences in morphology and the electrophoretic pattern of glycoproteins may influence the development of Trypanosoma cruzi in the intestinal tract of the insect.