ESA Annual Meetings Online Program

Function of ilp2 in honey bee larvae

Tuesday, November 15, 2011: 2:11 PM
Room D7, First Floor (Reno-Sparks Convention Center)
Ying Wang , School of Life Sciences, Arizona State University, Tempe, AZ
Sergio Azevedo , Biologia Celular e Molecular e Bioagentes Patogenicos, Universidade de São Paulo-Faculdade de Medicina de Ribeira˜o Preto, Avenida Bandeirantes, Brazil
Gro Amdam , School of Life Sciences, Arizona State University, Tempe, AZ
Robert E. Page , School of Life Sciences, Arizona State University, Tempe, AZ
Klaus Hartfelder , Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil
Nutrient signals play very important roles in queen and worker caste development in the honey bee. It has been suggested that insulin peptides (ilps) were involved in caste development in larvae. In this study, we knocked down ilp2 in larvae and monitored the larvae development and physiology. We found ilp2 was successfully down regulated by dsRNA. Surprisingly, glucose and trehalose titers were not altered by ilp2 knockdown. There was no effect of ilp2 gene knockdown on total mortality and body mass of adult bees. However, the ovary size was significantly reduced by dsRNA. Our study demonstrated that the function of ilp2 in the differentiation of ovary size.