The in vivo transcriptional regulation of Aedes aegypti sterol carrier protein-2 gene

The in vivo study of Aedes aegypti SCP-2 (AeSCP-2) promoter is the important aspect of exploring SCP-2 gene expression and regulation in the yellow fever mosquito. The promoter/reporter constructs with serial deleted AeSCP-2 promoter sequence using chloramphenicol acetyltransferase gene (CAT) as the reporter gene were transiently introduced into the G0 generation mosquitoes. Reporter gene products were determined in the midgut and the fat body samples from G0 mosquitoes, the promoter sequence between -1.3kb and -1.6kb upstream of 5’ flanking region of the AeSCP-2 gene is demonstrated to be critical for the tissue-specific promoter activity. In the pull-down assay, which aims to find the specific binding proteins to the promoter sequence, several binds were identified from the nuclear extract of the 4th instar larval midgut. The identification of the specific proteins binding to this region would help to illustrate the SCP-2 gene regulation.