0304 Functional analysis and molecular characterization of two acetylcholinesterases from the German cockroach, Blattella germanica

Monday, December 13, 2010: 10:32 AM
Sunrise (Town and Country Hotel and Convention Center)
Young-Ho Kim , Department of Entomology, Kansan State University, Manhattan, KS
Jae Young Choi , Seoul National University, Research Institute for Agriculture and Life Sciences, Seoul, Seoul, Korea, Republic of (South)
Yeon Ho Je , Department of Agricultural Biotechnology, Seoul National University, Seoul, Seoul, Korea, Republic of (South)
Young Ho Koh , Ilsong Institute of Life Science, Hallym University, Anyang, Korea, Republic of (South)
Si Hyeock Lee , Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea, Republic of (South)
Two acetylcholinesterases (AChEs; BgAChE1 and BgAChE2) from Blattella germanica were functionally expressed using the baculovirus system. Kinetic analysis demonstrated that BgAChE2 had higher catalytic efficiency but lower substrate specificity than BgAChE1. Except paraoxon, BgAChE1 was generally less sensitive to inhibitors than BgAChE2. Western blot analysis using anti-BgAChE antibodies revealed that BgAChE1 was far more abundant in all examined tissues compared to BgAChE2, which is only present in the central nervous system. Both BgAChEs existed in dimeric form, covalently connected via a disulfide bridge under native conditions. Most fractions of BgAChE1 had a glycophosphatidylinositol (GPI) anchor, but a small fraction comprised a collagen-like tail. BgAChE2 appeared to have a collagen-GPI-fused tail. Based on the kinetic and molecular properties, tissue distribution and abundance, BgAChE1 was confirmed to play a major role in postsynaptic transmission.

doi: 10.1603/ICE.2016.50516