D0684 Deciphering aphid behaviour to evaluate phytoviruses epidemiological riskssebastien.bocquel

Wednesday, December 15, 2010
Grand Exhibit Hall (Town and Country Hotel and Convention Center)
Sébastien Boquel , Biologie des Plantes et contrôle des Insectes ravageurs (UPRES EA-3900), Université de Picardie Jules Verne, Amiens, France
Charles Vincent , Horticultural Research and Development Centre, Agriculture & Agri-Food Canada, Saint-Jean-sur-Richelieu, QC, Canada
Philippe Giordanengo , Biologie des Plantes et Contrôle des Insectes Ravageurs, Université de Picardie Jules Verne, Amiens, France
Arnaud Ameline , UMR CNRS 7058 Edysan, Université de Picardie Jules Verne, Amiens, France
Non-persistent potato virus Y (PVY) transmitted by aphids causes serious damage to several cultivated plant species. From 2006 to 2009, transient aphids represented 80 to 90% of aphids trapped in potato fields of Picardie (northern France). These aphids were mainly cereal, crucifer and legume aphids, most likely associated to neighboring habitats of monitored potato fields. PVY transmission efficiency by aphids is related to the early steps involved in host plant selection process (i.e. superficial intracellular brief stings by stylets after landing on hosts) and to interplant movement ability. To evaluate the potential role of these different species in PVY epidemics we investigated, through laboratory experiments, the host plant selection process in potato aphids (Myzus persicae, Macrosiphum euphorbiae), two cereal aphids (Sitobion avenae, Rhopalosiphum padi), the pea aphid Acyrthosiphon pisum, the black bean aphid Aphis fabae and the cabbage aphid Brevicoryne brassicae. A dual choice test was developed to evaluate the aphid interplant movements and plant probing ability. All the tested species initiated a host plant selection on one plant. Only transient aphids were able to realize interplant movements. The electrical penetration graph (EPG) technique was used to investigate aphid-probing behaviour on potato plants. All the species realized superficial intracellular brief stings (i.e. potential drops) involved in PVY transmission, only colonizing potato species ingesting phloem sap. The use of aphid behaviour as a predictor of phytoviruses spreading is discussed.

doi: 10.1603/ICE.2016.49131