Biological evaluation of a watery and oil extract of neem on the entomopathogenic bacterium Bacillus thuringiensis

Nowadays, the use of chemical pesticides is very common in the control of agricultural and domestic pests, ignoring the environmental impact that causes an irrational use of these products in the ecosystem. In our work we investigated alternative bioinsecticides that have a little environmental impact. Bacillus thuringiensis, a bacterium with natural insecticide activity has been demonstrated to have high potential for the control of insects. Similarly, the neem extracts show favorable characteristics as they have a little impact on non-traget organisms such as natural enemies of insect pests. The combination of Neem and Bacillus thuringiensis may affect the physiology of the insect pest which allows us to control their populations. We have evaluated the possibility of use the combination for increase the insecticide effect in comparasion with each component separately. However, it is also necessary to verify if the combination of the extracts of neem does not generate an inhibition effect on the entomopathogen bacterium. In this aspect we evaluated eight different doses, 0, 0.0625, 0.125, 0.25, 0.5, 1, 2 and 4% in liquid and solid mediums. None of the concentrations show an antagonist effect on the bacetrium.