Diversity of CYP genes in Dendroctonus rhizophagus and Dendroctonus vales in presence of α-pinene

Phylogenetic studies showed that Dendroctonus rhizophagus and D. valens had a narrow relationship, however biological characteristics indicate differences between both species. The bark beetle D. rhizophagus is endemic of northwestern Mexico, its considered a primary species that colonize only young trees, while, D. valens has a broad geographical distribution in North America and its considered a secondary bark beetle that colonize around 40 different host. During their life cycle and initial attack on a living tree the insects are exposed to huge concentrations of host-plant-produced defensive toxins. Like other insects, bark beetles probably overcome these defenses partially through enzyme-mediated detoxification of host compounds. Cytochrome P450 (CYP) enzymes are well known for their role in biosynthesis of endogenous compounds as well as in xenobiotic metabolism. The objective of this work is know the diversity of CYP genes of the CYP3 and CYP4 clades, when these species are exposed with alpha-pinene the principal monoterpene of the resin. The analysis the expression of these genes was carried out by RT-PCR in antennae and the alimentary canal (foregut, midgut and hindgut) total RNA from insects stimulated with a-pinene, (S)-(-)a, (S)-(-)b, (R)-(+)a enantiomers. We found genes 6 and 9 of the clade 3 and variants of CYP4 clade, expressed in all the anatomical regions with different stimuli. Different genes were not found in both species. The sequences were highly similar con Ips paraconfusus and Tribolium castaneum.