mtDNA barcoding for taxonomic identification within the genus Agrilus

Species identification in the genus Agrilus is difficult, and often requires dissection and examination of the male adeagus. This method can, however, exclude both females and larvae from positive identification. Ecological studies that rely on accurate species identification may become complicated and time consuming due to these taxonomic difficulties. Furthermore, increases in the number of invasive species highlight the need for faster identification of potentially harmful exotics. Recent studies have shown the efficacy of mtDNA barcoding to differentiate morphologically similar species. I used a 658bp fragment internal in the cytochrome c oxidase I (COI) gene to evaluate the effectiveness of this technique within the genus Agrilus. Results indicate that mtDNA barcoding using the COI gene will provide a viable method to distinguish between species in the genus.