1054 Analysis of transcriptional activity of the AeSCP-2 promoter in Aedes aegypti mosquito cell line culture

Wednesday, November 19, 2008: 8:29 AM
Room A1, First Floor (Reno-Sparks Convention Center)
Irina Vyazunova , Entomology, University of Wisconsin-Madison, Madison, WI
Que Lan , Department of Entomology, University of Wisconsin, Madison, WI
AeSCP-2, a sterol carrier protein, is involved in sterol trafficking in mosquitoes. The activity of the AeSCP-2 gene is important for mosquito development (Blitzer et al. 2005). An earlier study demonstrated that the transcription of this gene was up-regulated by 20-hydroxyecdyson in cultured gut tissues. To investigate 20E-regulated transcription of AeSCP-2 gene we truncated the upstream flanking region of AeSCP-2 gene and linked them to a reporter gene. The mosquito Aag-2 cell line was transfected with these promoter/reporter constructs and treated with â-ecdysone at various concentrations. Expression vectors of different transcription factors such as MHR3 and âFTZ-F1 were also co-transfected with the AeSCP-2 promoter/reporter constructs. The observed results demonstrated that varied combinations of transcription factors produce different promoter activities of the AeSCP-2 gene. This observation leads us to the conclusion that the partnership of transcription factors is crucial in regulating transcriptional activity of AeSCP-2 gene.

doi: 10.1603/ICE.2016.37435