Patterns of gas exchange among terrestrial arthropods are highly variable and range from continuous through to discontinuous with discretely partitioned phases. The underlying generation and subsequent co-ordination of these patterns remains relatively poorly understood and here we present a novel method for simultaneous measurement of ventilatory nervous innervation by the CNS, VCO2 and evaporative water loss in medium to large sized species (> 0.5 g). Preliminary data show consistent results of high resolution and low noise that permit minimally invasive visualization of nervous output relative to typical resting gas exchange data for the first time. The location of central pattern generators for ventilation can be assessed, in addition to inferring the presence of localized oxygen and/or carbon dioxide sensors at different locations in CNS. Excellent potential exists for insight into how patterns of gas exchange are regulated relative to temperature and modified atmospheres as well as in more applied chemical and drug applications.