Terrestrial slugs (Pulmonata) have economic and ecological consequences in urban, agricultural, and woodland environments. They are pests in nurseries and gardens in Kentucky and, by eating young plants, slugs slow the growth of new deciduous forests for plant restoration projects. To implement successful management programs, including the use of invertebrate natural enemies in biological control, it is necessary to ascertain the faunistic composition of mollusks within these environments. Therefore, Kentucky’s first comprehensive statewide evaluation of native and invasive slug fauna was conducted in 2007. Collection sites included residential lawns, plant nurseries, state parks, woodlands, and the University of Kentucky Arboretum. Collection methods included hand-collecting slugs from under logs, rocks, landscaping timber, and baited traps. The findings of this evaluation included species of Arionidae previously undocumented in Kentucky and several new county records. The survey revealed large populations of Arion hortensis, Arion intermedius, Arion subfuscus, Deroceras laeve, Deroceras reticulatum, Lehmannia valentiana, Limax maximus, and Philomycus sp. and several natural biocontrol agents overlapped in distribution with these invasive pest species. This information provides insight into the ecology and potential impact of slugs in Kentucky, and is useful to land-managers and nursery professionals who can utilize such information during management programs.