The phylogenetic relationships within the subfamily Sarcophaginae, the largest of the three subfamilies of the Sarcophagidae, were studied using Cytochrome Oxidase I (COI), parts of Cytochrome Oxidase II (COII) and Subunit four of the Dehydrogenase complex (ND4) for twenty-one species of flesh fly in order to investigate generic organization for this group. Our investigation supported the generic relationships for the Ravinia, Sarcophaga, Peckia, Helicobia and Boettcheria groups as organized in the current world catalogue. Our data also revealed a close relationship between the Ravinia and Helicobia genera. Our data did not resolve any deep interordinal relationships.