Tuesday, December 12, 2006

Alkaline phosphatase as a biomarker for resistance to Bacillus thuringiensis toxins

Juan Luis Jurat-Fuentes, jurat@utk.edu, University of Tennessee, Dept of Entomology & Plant Pathology, 2431 Joe Johnson Drive, 205 Ellington Plant Sciences Building, Knoxville, TN, Stephen F. Garczynski, sgarczynski@yarl.ars.usda.gov, USDA-ARS, Yakima Agricultural Research Laboratory, 5230 Konnowac Pass Road, Wapato, WA, William J. Moar, moarwil@auburn.edu, Auburn University, 301 Funchess Hall, Auburn, AL, and Michael J. Adang, adang@uga.edu, University of Georgia, Dept of Entomology, 413 Bio Sci Bldg, Cedar St, Athens, GA.

We previously reported identification of an alkaline phosphatase (HvALP) that bound Cry1Ac toxin in brush border membrane vesicles (BBMV) from Heliothis virescens. Both alkaline phosphatase-specific activity and HvALP protein levels were reduced in Cry1Ac-resistant larvae from three independent Cry1Ac-resistant strains of H. virescens. Similarly, reduced alkaline phosphatase activity was detected in BBMV from alterations in alkaline phosphatase as a marker for resistance to Cry toxins, at least for these two insect species.

Species 1: Lepidoptera Noctuidae Heliothis virescens (tobacco budworm)
Species 2: Lepidoptera Noctuidae Spodoptera exigua