Dendroctonus ponderosae (Coleoptera, Scolytidae), D. brevicomis (Coleoptera, Scolytidae), and Ips pini (Coleoptera, Scolytidae) adults were placed into bolts of Pinus contorta or P. ponderosa and removed at intervals to determine the longevity/survival of phoretic and mycangial fungal associates in the genus Ophiostoma. The beetles were rinsed in sterile de-ionized water and the water plated on an Ophiostoma-selective malt agar. From these plates, estimates were made of viable phoretic spores over time, as measured from time of attack. In addition, glandular mycangia of D. brevicomis were dissected out and plated on selective agar to qualitatively assess the presence or absence of viable Ophiostoma. The ability of beetles to retain viable fungal spores over time may have important management implications: average annual temperatures have increased throughout ranges of the three beetle species, so there is potential for an increased number of generations per year and for adults to re-emergence after an initial penetration to attack additional trees.