Wednesday, December 13, 2006
D0410

Cloning and characterization of a salivary digestive lipase from Hessian fly (Diptera: Cecidomyiidae)

Richard Shukle, shukle@purdue.edu1, Omprakash Mittapalli, omittapa@purdue.edu2, Ming-Shun Chen, mchen@ksu.edu3, and Alisha Johnson, johnso27@purdue.edu1. (1) USDA-ARS, Purdue University, Entomology, 901 West State Street, West Lafayette, IN, (2) Purdue University, Entomology, 901 West State Street, West Lafayette, IN, (3) Kansas State University, Department of Entomology and USDA-ARS-PSERU, 123 Waters Hall, Manhattan, KS

Secreted digestive lipases have been reported to be virulence factors in fungal pathogens. Here, we report the identification of a putative secreted digestive lipase from larval Hessian fly. Analysis by quantitative real-time PCR of temporal and spatial mRNA levels indicates the lipase is expressed in salivary glands of feeding larvae. The role of the lipase in the Hessian fly/wheat interaction is currently being revealed.


Species 1: Diptera Cecidomyiidae Mayetiola destructor (Hessian fly)