The soybean aphid, Aphis glycines Matsumura, is a soybean pest native to Asia, and it has recently become a principal pest of soybeans in many areas of the United States and Canada. Current management practices rely on insecticides, but host-plant resistance is a potential, alternative management tool for A. glycines. Growth-chamber tests were conducted to determine and characterize resistance among accessions of soybean to A. glycines. Screening tests identified accessions resistant to A. glycines, and follow-up experiments showed that resistance could be characterized as antixenosis and antibiosis. Our results suggest that at least two accessions with newly identified resistance to A. glycines are potentially useful for soybean breeding programs.