The woolly whitefly, Aleurothrixus floccosus (Maskell) is a serious pest of citrus trees worldwide. Like other whiteflies, these insects cause direct damage to leaves by sucking phloem sap and producing honeydew, which facilitate the growth of black sooty mold. Ants are attracted to honeydew and may interfere with natural control of whiteflies and other hemipteran pests. Efforts to eradicate woolly whitefly using chemical insecticides were unsuccessful. Consequently, management efforts have focused on biological control of woolly whitefly in countries where it has become a pest. The parasitoid wasp, Cales noacki Howard, is one of the most important biological control agents worldwide. Despite its extensive use for biological control, much systematic work remains to be done on the genus Cales (and the subfamily Calesinae). There is no consensus regarding placement of this subfamily, although it is most often associated with the Aphelinidae. DNA sequence analyses of C. noacki suggest that at least three distinct haplotypes coexist in the biocontrol citrus grove at the University of California, Riverside. One of the haplotypes matches collections from Italy and also from redbanded whitefly on in California Avocado groves. The second haplotype is known only from the biological control grove and has enough nucleotide differences to be considered as not just another reproductively isolated species, but as compared to other Chalcidoidea, possibly another genus. Here, we present a detailed analysis of the C. noacki species complex as well as its biogeographic distribution in California.