The pepper weevil, Anthonomus eugenii Cano, is a major pest of all varieties of cultivated peppers. The weevils feed and oviposit on flower buds and young fruits, resulting in bud drop and a decrease in yield. After laying an egg in a feeding puncture, usually near the fruit calyx, females apply a clear yellow anal over the oviposition scar that hardens into a plug. This plug is often covered with a small amount of frass. Behavioral bioassays demonstrated that female weevils laid fewer eggs in fruits that contained eggs laid by themselves or by conspecifics. Oviposition plugs applied to clean, uninfested fruit deterred further oviposition. We also found deterrent activity in female frass but not male frass when it was applied around the calyx of clean fruit. Additionally, removal of plugs and/or frass from infested fruit increased ovipostion. In order to isolate the deterrent component, oviposition plugs were extracted in a series of solvents (water, methanol:water (50:50), methanol:water (80:20) and methylene chloride). Twenty oviposition plug equivalents of extract (5uL) were applied around the calyx of a pepper fruit. Eleven treatments (4 extracts, recombined extract, pepper with 5 oviposition plugs applied, 4 solvent controls, and clean pepper control) were evaluated under no choice conditions. Individual gravid females were allowed to oviposit on the fruit for 12 h. The number of eggs laid per female for each treatment was analyzed. Deterrent fractions will be subjected to column and HPLC fractionation in order to identify active compounds.