Wednesday, December 13, 2006
D0412

Quantification of azadirachtin extracted from neem leaves using HPTLC separation/development and image J analysis

Donald Mullins, mullinsd@vt.edu, Lisa Moore, lmmoore@vt.edu, Sandra Gabbert, sgabbert@vt.edu, and Richard D. Fell, rfell@vt.edu. Virginia Tech, Department of Entomology, 319B Price Hall, Virginia Polytechnic Institute and State University, Blacksburg, VA

A High Performance Thin-Layer Chromatographic Technique (HPTLC) has been developed for separation and quantitative determination of azadirachtin obtained from neem extracts. Traditional separation and detection procedures are coupled with quantification using Image J analysis of the detected plates. This system provides a high level of accuracy and sensitivity utilizing a minimal amount of equipment that could find application in laboratories with limited resources.