The genus Epitheca (Odonata:Corduliidae) is composed of about 13 species and several named subspecies of uncertain validity. The North American members of Epitheca are placed in the subgenus Tetragoneuria and the monotypic subgenus Epicordulia. The status and relationships among and within subgenera of Epitheca are currently unresolved, with some believing the subgenera should be elevated to genus level. Additionally, morphological variation within the species E. (T.) cynosura, E. (T.) costalis, E. (T.) petechialis, and E. (T.) semiaquaea across geographic regions make the species difficult to determine. This analysis of sequences retrieved from the COI region of mitochondrial DNA and the D2 region of 28S large subunit nuclear ribosomal RNA provides clarification to the status of Tetragoneuria as well as providing insight to the species-level ambiguities within the genus.