The Japanese beetle, Popillia japonica Newman, is a voracious feeder on turfgrasses and landscape plants in the United States. Biological control approaches have included use of the entomopathogenic fungus Metarhizium anisopliae (Metsch.) Sorokin. Auto-dissemination chambers have been used by state agriculture departments to inoculate adults that would then potentially disseminate conidia to other adults, and to soil microcosms and infect larvae. Conidia of M. anisopliae ESC-1 were evaluated for virulence against adults, eggs, and all larval instars of Japanese beetles under laboratory conditions. Two inoculum methods, conidia within agar plates and formulations of conidia blended with corn starch, were used. The infectivity and mortality of M. anisopliae for adult Japanese beetles was used to estimate the potential for dispersal of the pathogens to the soil which would subsequently affect egg hatch and larval survival. Egg and larval dose-mortality relationships were used to determine if sufficient inoculum was accumulated by adults in auto-disseminator chambers to negatively affect other life stages (eggs and larvae) of the Japanese beetle.