The development of the biopesticide-based control Beauveria bassiana (BB) may help strengthen a multi-tactic approach to manage plum curculio (PC), Conotrachelus nenuphar, in reduced risk and organic management systems. The entomopathogenic fungus BB offers the opportunity to attack previously untargeted PC life stages occupying the soil including late instar larvae, pupae and eclosed adults. The pathogen demonstrated some success in limited 2005 field ground spray trials on MI tree fruit farms. In the 2006 study, different application methods were tested to deliver and provide inoculum for long periods of time. In a randomized block design five treatments (three inoculation rates of BB on rice, Mycotrol-O spray and no-treatment control) were carried out which yielded 15 plots sealed under ground emergence traps for each of 3 orchard sites. Ten newly emerged PC larvae were placed into the litter of each of four soil plugs inserted into plastic containers sealed under a fitted emergence trap. Captured emerged adult PC and recovered PC cadavers were evaluated microscopically after lab incubation. The quantification of BB establishment was also tested using soil samples from different treatments.