Biological invasions due to human introductions and species extinctions due to habitat loss pose two of the most serious challenges to environmental sustainability. We know particularly little about management options where these two issues intersect. The gypsy moth, Lymantria dispar (Linnaeus), is managed at the western edge of its invasion by a variety of methods, with selection based on efficacy, economics, and potential impacts on nontarget species. In Wisconsin, both the endangered Karner blue butterfly, Lycaeides melissa samuelis (Nabokov), and several sphingid pollinators of the endangered Eastern prairie fringed orchid, Platanthera leucophaea (Nutt.) Lindl., live in vanishing habitats either bordering or overlapping the gypsy moth’s range. To control gypsy moth in such ecologically sensitive areas Gypchek, a formulation of nuclear polyhedrosis virus, is used to minimize nontarget impacts. Gypchek is applied in conjunction with a viscous molasses-based carrier (Carrier 038-A), which acts as a surfactant and a sunscreen. While the nontarget impacts of other gypsy moth controls have been explored, the effects of Gypchek in conjunction with its carrier on the Karner blue butterfly and sphingid pollinators are unknown. We performed laboratory bioassays to determine if Gypchek and its carrier would have an effect on the mortality of both the larvae of the Karner blue butterfly and of Manduca sexta (Linnaeus), which was used as a surrogate for the sphingid pollinators. Carrier 038-A alone, as well as in conjunction with Gypchek, had negative but relatively weak effects on both the Karner blue butterfly and Manduca sexta.