To search for patterns in the distribution of imidacloprid throughout trees, two experiments were conducted. In the first, the roots of dormant saplings were soaked in an imidacloprid solution for four days and then planted in a greenhouse until full leaf development, about three months. The different plant tissues, the leaves, stems, and roots were analyzed separately for pesticide residue. Based on published data, we expect the leaves to have a higher pesticide residue level than either the stem or the roots. In the second experiment, three trees treated with imidacloprid, two by soil injection and one by trunk injection, were intensively sampled and samples were compared for patterns in distribution. Twelve samples were taken from each tree; each sample consisting of leaves, twigs, and in two trees, fruiting bodies. Samples were taken at three different heights and from four different sides of the tree. We expect to find some variation between samples from the same tree but not to find a specific pattern due to height or side of the tree. All samples from both experiments were dried, ground, extracted in methanol, and analyzed for pesticide residue using a commercially available ELISA assay. Results thus far support our expectations, with the exception of high residue levels found in some roots.