Using a dual-choice olfactometer, certain bird-associated odors elicited attraction of host-seeking Culex quinquefasciatus females in the absence of carbon dioxide, whereas controls did not. Head space samples were collected using solid phase micro-extraction (SPME). Using electroantennograms coupled with gas chromatography, eight novel host-associated odors were identified from the extracts, and were found to elicit antennal responses in electroantennograms of Cx. quinquefasciatus females, but not males. An improved technique for electroantennograms is introduced in which four antennae were tested simultaneously.