Brook Creek, located near Dorchester in extreme Northeastern Iowa, was returned to its original streambed in September 2004 as part of a large habitat restoration project. This study compares the aquatic macroinvertebrate communities of Brook Creek 8-11 months after restoration with three other trout streams in NE Iowa. Six riffles within Brook Creek, South Pine Creek, and Waterloo Creek, and three riffles in Duck Creek were sampled for aquatic macroinvertebrates in late May, mid-June, mid-July, and late-August 2005. Samples were collected using a 12 x 12" Surber sampler and stored in 70% ethanol until sorted, counted, and identified to family level in the lab. Overall, 19,690 macroinvertebrates were collected from the four streams representing 39 different taxonomic families, with an average density of 234.4 macroinvertebrates/ft2. South Pine Creek had the greatest overall taxonomic richness (n=31) while Brook Creek had the lowest overall taxonomic richness (n=18). During each sampling period, among the four streams there were significant differences in the overall number of taxa (P<0.001) as well as the insect density (P<0.001). The primary taxonomic groups noticeably absent from Brook Creek in comparison with the established streams were several families of caddisflies. It is expected that the taxonomic richness of Brook Creek will increase as the stream becomes reestablished.