Extensive information is available on diversity of tropical insect within a single site; however, data on species turnover across large areas of continuous forest (beta-diversity) are almost nonexistent. We sampled fauna of ambrosia beetles (Xyleborina & Platypodinae; Curculionidae - Scolytinae) at three sites forming 2000 km transect of continuous lowland tropical rainforest in Papua New Guinea. At each site, three specimens of each of the four sampled tree species were artificially killed and dissected to collect the attacking ambrosia beetles with equal sampling effort. Our results show that neither the species assemblage nor the community structure changes significantly within 2000km. Assemblages of ambrosia beetles attacking tree trunks (the majority) differed neither between localities nor between hosts. Only the assemblage attacking branches and twigs seem to be marginally site- and host-specific. Also bispecific host-parasite ambrosia beetle associations remain identical over large geographical distances.