The 2005 ESA Annual Meeting and Exhibition
December 15-18, 2005
Ft. Lauderdale, FL

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Thursday, December 15, 2005 - 10:25 AM
0036

Mixtures and mealybugs: Response surface methodology for development and optimization of insect diets

Terence Evens, tevens@ushrl.ars.usda.gov, Stephen L. Lapointe, slapointe@ushrl.ars.usda.gov, and Randall Niedz, rniedz@ushrl.ars.usda.gov. USDA, ARS, USHRL, 2001 South Rock Road, Fort Pierce, FL

Insect diet development can be difficult and time-consuming because of the large number of permutations of diet components to be tested. One-factor at a time (OFAT) approaches are time, labor and cost prohibitive and are inherently confounded. However, factorial mixture designs provide a powerful and relatively simple approach to de-convoluting the effects and interactions of multiple diet components. Screening designs facilitate the analysis of up to 24 components in a single experiment, while crossed, full D-Optimal mixture designs are capable of quantifying the interactions/effects of 6 or more components across a temperature range. The response surfaces generated by this approach can be used to define numerous diet ≥optima≤ as functions of multiple responses, such as development time, sex ratios, and/or days to adult emergence. Mixture response surface methodology, as it relates to insect diet formulations and component screening, will be discussed along with the results and implications of a newly developed medium for the pink hibiscus mealybug Maconellicoccus hirsutus.


Species 1: Hemiptera Pseudococcidae Maconellicoccus hirsutus (pink hibiscus mealybug)
Keywords: artificial diet

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