The 2005 ESA Annual Meeting and Exhibition
December 15-18, 2005
Ft. Lauderdale, FL

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Thursday, December 15, 2005 - 10:25 AM

Mixtures and mealybugs: Response surface methodology for development and optimization of insect diets

Terence Evens,, Stephen L. Lapointe,, and Randall Niedz, USDA, ARS, USHRL, 2001 South Rock Road, Fort Pierce, FL

Insect diet development can be difficult and time-consuming because of the large number of permutations of diet components to be tested. One-factor at a time (OFAT) approaches are time, labor and cost prohibitive and are inherently confounded. However, factorial mixture designs provide a powerful and relatively simple approach to de-convoluting the effects and interactions of multiple diet components. Screening designs facilitate the analysis of up to 24 components in a single experiment, while crossed, full D-Optimal mixture designs are capable of quantifying the interactions/effects of 6 or more components across a temperature range. The response surfaces generated by this approach can be used to define numerous diet ≥optima≤ as functions of multiple responses, such as development time, sex ratios, and/or days to adult emergence. Mixture response surface methodology, as it relates to insect diet formulations and component screening, will be discussed along with the results and implications of a newly developed medium for the pink hibiscus mealybug Maconellicoccus hirsutus.

Species 1: Hemiptera Pseudococcidae Maconellicoccus hirsutus (pink hibiscus mealybug)
Keywords: artificial diet

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