The braconid subfamily Alysiinae is a large group of dipteran endoparasitoids with over 1,000 species worldwide. Dacnusini is one of two tribes in Alysiinae, and 15 to 25 genera have been recognized. There is disagreement on how certain dacnusine genera should be defined using morphological data. For example, Chaenusa sensu lato currently contains all dacnusines with setiferous compound eyes, but wing venation has also been used to divide the group into three smaller genera. While there is disagreement on the definition of Chaenusa using morphological data, phylogenetic analyses using molecular data have not been undertaken. In this study DNA sequences from the mitochondrial NADH 1 dehydrogenase gene were used to assess the monophyly of Chaenusa s. l. and determine if support exists for any of the three previously recognized genera. Preliminary parsimony analysis indicates that Chaenusa s. l. is not monophyletic, while the data support two of the three previously recognized genera as monophyletic groups.