Monday, 15 November 2004 - 9:06 AM
0043

Molecular cloning and characterization of two digestive serine proteases from the first-instar larvae of the Hessian fly, Mayetiola destructor (Say)

Omprakash Mittapalli, omitt@purdue.edu1, Jeff Stuart, jeff_stuart@entm.purdue.edu1, and Richard Shukle, shukle@purdue.edu2. (1) Purdue University, Entomology, 901 W. State Street, Smith Hall, West Lafayette, IN, (2) Purdue University, USDA-ARS, Dept. of Entomology, Smith Hall, 901 W. State Street, West Lafayette, IN

Full-length cDNA and genomic DNA sequences for two genes (designated mdesprot-I and mdesprot-II) encoding digestive serine proteases from the first-instar larvae of Mayetiola destructor have been cloned and characterized. These results indicate the mdesPROT-I and mdesPROT-II proteins are secreted proteases that most likely function as midgut digestive proteases. A cytological analysis with Fluorescent in situ hybridization revealed the localization of mdesprot-I and mdesprot-II on the long arm of Autosome 2. An EST project directed toward analyzing gene expression of midgut transcripts is currently being pursued. These results will develop microarrays for analysis of gene expression during compatible vs. incompatible interactions as well as the effects of transgenes for resistance on the Hessian fly midgut.


Species 1: Diptera Cecidomyiidae Mayetiola destructor (Hessian fly)
Keywords: digestive serine-protease, Hessian fly-wheat interaction

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