Ecdysteroids are synthesized by ovaries of female Aedes aegypti following a blood meal, which promotes fat body production of vitellogenin. The full ecdysteroid biosynthetic pathway is not known, but various proteins involved in the biosynthetic process have been identified by analysis of Drosophila melanogaster ecdysteroid mutants and by biochemical and molecular techniques in other insects. Previously, seven genes were cloned from Aedes aegypti ovaries that code for proteins that would be responsible for both the intracellular transport of ecdysteroid precursors and enzymes involved in their biosynthesis. Gene transcripts for all seven genes were observed in tissues other than the ovaries both before and following a blood meal. The various tissues in which the seven gene transcripts were observed produced significantly less ecdysteroids than ovaries following a blood meal. The lack of distinctive tissue and temporal transcript expression of some of the gene transcripts contradicts previous research reported for D. melanogaster and Bombyx mori. Nonetheless, real-time PCR analysis showed an increase in transcript abundance within the ovaries for three of the biosynthetic enzymes during peak ecdysteroid production post blood meal, and the transcripts for one “transporter” displayed a substantial increase later in the gonotropic cycle.