The effect of jasmonic acid (JA) on the resistance in cotyledon of sweet pepper seedling against leafminer (Liriomyza trifolii) was investigated. After the cotyledons were floated in the JA aqueous solution for 48 hours, they were exposed to oviposition of leafminer. The cotyledons treated with JA at the concentration over 50 mM showed resistance against leafminer. Comparing the secondary metabolites in the treated cotyledon with that in the untreated cotyledon by HPLC, an enhanced peak was observed. This peak was purified with octadesyl silica gel column and reversed phase HPLC, and identified as caffeoylputrescine by measuring LC-MS and NMR.
Species 1: Diptera Agromyzidae Liriomyza trifolii (leafminer, legume leafminer)
Keywords: oviposition inhibitor, hydroxycinnamate
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