Wednesday, 29 October 2003
D0488

This presentation is part of : Display Presentations, Section B. Physiology, Biochemistry, Toxicology, and Molecular Biology

Comparative analysis of mosquito ribosomal protein RPS3

Lei Li and Ann M. Fallon. University of Minnesota, Entomology, 1980 Folwell Ave, St. Paul, MN

Ribosomal protein S3 (RPS3) is a structural component of the ribosome that also plays a role in DNA repair. Here we used RT-PCR to clone rpS3 cDNA from Aedes albopictus C7-10 mosquito cells. We also used GeneRacer kits to clone rpS3 cDNA from Aedes aegypti cells. The deduced translation products from these cDNA clones were further compared with RPS3 from Anopheles gambiae. The results revealed that RPS3 is well-conserved in amino acid sequence, consistent with its role as a ribosomal protein and as a DNA repair enzyme.

Species 1: Diptera Culicidae Aedes albopictus (Asian tiger mosquito)
Species 2: Diptera Culicidae Aedes aegypti (yellow fever mosquito)
Species 3: Diptera Culicidae Anopheles gambiae (malaria mosquito)
Keywords: ribosome, DNA repair

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