Multicolored Asian lady beetle (H. axyridis, Col: Coccinellidae) is a successful biological control agent against aphid and scale damage. Recognizing temperature responses of predatory insects is an important part of predicting their potential as bio-control agents of herbivorous insects.  In this study we used isothermal calorimetry to measure metabolic heat and CO₂ production rates of multicolored Asian lady beetle pupae as a function of temperature.  Metabolic heat rates (R_q) and respiration rates (R_CO2) of pupae were measured in isothermal calorimeters at 5°C intervals from 0 to 35°C.  Anabolic rates and energy use efficiencies were calculated as functions of temperature from the calorespirometric data.  The respiration-based calculations of isothermal calorimetry were consistent with known behavior of lady beetles at corresponding temperatures.  The results demonstrate the energy use efficiency of lady beetles changes continuously with temperature.  Calorespirometry is fast and less labor intensive and proves to be a valuable aid in determining the correct match between climate and physiological niche for insects that have agricultural importance.