A 1962 bp cDNA clone of 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (purH) was isolated from diapausing adults of Leptinotarsa decemlineata using RT-PCR and 3' and 5'-RACE. The probes generated from this clone hybridized to a transcript approximately 1960 bp in length on northern blots. The clone encodes for a deduced protein 594 amino acids in length with 73% identity, 83% similarity to purH from Drosophila melanogaster. Northern blot analysis determined that L. decemlineata purH (LdpurH) was downregulated in diapausing beetles. Developmental studies revealed that LdpurH is expressed at nearly constant high levels in both nondiapausing and prediapause adults. LdpurH expression decreases over the course of diapause development from levels equivalent to those seen in nondiapausing beetles.
Species 1: Coleoptera Chrysomelidae Leptinotarsa decemlineata (Colorado potato beetle)
Keywords: Diapause nucleotide
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