We had reported the cloning of a 5-hydroxytryptamine receptor (Aedes 5-HT7-like receptor) from adult Aedes aegypti. For functional expression of the Aedes 5-HT7-like receptor, CHO-K1 cells were stably transfected with a receptor expression construct, pC5-HT7. The Aedes 5-HT7-like receptor positively coupled to Gs protein, increasing intracellular cAMP in response to 5-HT; adenylyl cyclase activity was induced in a concentration-dependent, saturable manner. Only 5-HT and not octopamine, dopamine or tyramine, caused the induction of cAMP. Other known agonists of the mammalian 5-HT7 receptor were tested. This is the first report on the functional expression of a mosquito neurohormone receptor.
Species 1: Diptera Culicidae Aedes aegypti (yellow fever mosquito)
Keywords: G protein-coupled receptor, respiratory system
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