Differential expression of candidate salivary effector proteins in field collections of Hessian fly, Mayetiola destructor

Tuesday, June 2, 2015
Big Basin (Manhattan Conference Center)
Alisha Johnson , Entomology, USDA-ARS, Purdue University, West Lafayette, IN
Richard Shukle , Entomology, USDA-ARS, Purdue University, West Lafayette, IN
Ming-Shun Chen , Department of Entomology, USDA - ARS, Manhattan, KS
Sanvesh Srivastava , Statistical Science, Duke University, Durham, NC
Subhashree Subramanyam , Department of Agronomy, Purdue University, West Lafayette, IN
Brandon Schemerhorn , USDA-ARS and Department of Entomology, Purdue University, West Lafayette, IN
Christie E. Williams , Crop Production and Pest Control Research, USDA, Agricultural Research Service, West Lafayette, IN
Evidence is emerging that some proteins secreted by gall-forming parasites of plants act as effectors responsible for systemic changes in the host plant, such as galling and nutritive tissue formation. A large number of secreted salivary gland proteins (SSGPs) that are the putative effectors responsible for the physiological changes elicited in susceptible seedling wheat by Hessian fly, Mayetiola destructor (Say), larvae have been documented. However, how the genes encoding these candidate effectors might respond in field collections from different geographic regions is unknown. The goal of this study was to use microarray analysis to investigate variation in SSGP transcript abundance among field collections from different geographical regions (southeastern USA, central USA, and the Middle East). Results revealed significant variation in SSGP transcript abundance among the field collections studied. The field collections separated into three distinct groups that corresponded to the wheat classes grown in the different geographical regions as well as to recently described Hessian fly populations. These data support previous reports correlating Hessian fly population structure with micropopulation differences owing to agro-ecosystem parameters such as cultivation of regionally adapted wheat varieties, deploymentof resistance genes and variation in climatic conditions.
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