Olfactory mediated responses to host and non-host plant volatiles by female grape berry moths (Paralobesia viteana)

We investigated the behaviorally active host (grape) and non-host (apple, dogwood) plant volatiles to understand the proximate mechanism of olfactory mediated host plant discrimination by the specialist phytophagous insect, the grape berry moth (Paralobesia viteana). We collected headspace volatiles from live grape, apple, and dogwood shoots using activated charcoal traps and identified volatile compounds that can be detected by GBM antennae using a combination of gas chromatography coupled with electroantennographic detection (GC-­EAD) and GC­‐MS. We have determined an active blend derived from host plant volatiles that serves as an attractant to mated females in previous flight tunnel work. We will asses additional volatiles isolated from non-host plants in the flight tunnel to demonstrate how these compounds affect moth flight behavior.