Application of RNAi to control of tobacco whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) and target gene selection

Tuesday, November 17, 2015
Exhibit Hall BC (Convention Center)
Jeong Hee Kim , Chungnam National University, Daejeon, South Korea
Jung-Kyu Kim , Department of Applied Biology, Chungnam National University, Daejeon, South Korea
Chan-yeong Kang , Department of Applied Biology, Chungnam National University, Daejeon, South Korea
Yu-Bin Jung , Department of Applied Biology, Chungnam National University, Daejeon, South Korea
Ji Hyun Min , Chungnam National University, Daejeon, South Korea
Il Hyun Byun , Chungnam National University, Daejeon, South Korea
Hyun Ju Jang , Chungnam National University, Daejeon, South Korea
Min Gyu Cho , Chungnam National University, Daejeon, South Korea
Hyoun-Sub Lim , Department of Applied Biology, Chungnam National University, Daejeon, South Korea
Yong-Man Yu , Department of Applied Biology, Chungnam National University, Daejeon, South Korea
Young-Nam Youn , Department of Applied Biology, Chungnam National University, Daejeon, South Korea
 Tobacco whitefly, Bemisia tabaci, is major pest that feeds over 600 plants. The most general method to control of tobacco whitefly is chemical control using insecticides, but this method have resistance problem. So, in this study, we try to control tobacco whitefly using RNAi. First of all, to select target gene for using RNAi, we construct cDNA library of tobacco whitefly and conduct LR recombination to tobacco rattle virus (TRV) RNA2 vector added att site to induce RNAi. To select genes that have effect to control of tobacco whitefly, we inoculated random genes to plant, and induced feeding of tobacco whitefly. Consequently, three unknown genes were selected. So, we checked whether or not three genes expressed in plant and gene expression changed by RNAi in insect through qRT-PCR. It is suggested that final selected target gene may be applied to control of tobacco whitefly using RNAi.

Keyword: Bemisia tabaci, cDNA library, target gene, RNA interference

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