Discovery of midgut genes for the RNA interference control of corn rootworm

Tuesday, November 17, 2015: 9:50 AM
200 I (Convention Center)
Xu Hu , Plant Protection, DuPont Pioneer, Johnston, IA
Nina Richtman , Plant Protection, DuPont Pioneer, Johnston, IA
Lisa Procyk , Plant Protection, DuPont Pioneer, Johnston, IA
Jian-Zhou Zhao , Plant Protection, DuPont Pioneer, Johnston, IA
Meghan Oneal , DuPont Pioneer, Johnston, IA
Joe Steimel , Plant Protection, DuPont Pioneer, Johnston, IA
Xiping Niu , Plant Protection, DuPont Pioneer, Johnston, IA
Bliss Kernodle , DuPont Pioneer, Johnston, IA
Virginia Crane , Plant Protection, DuPont Pioneer, Johnston, IA
Jeff Robson , DuPont Pioneer, Johnston, IA
Gary Sandahl , Plant Protection, DuPont Pioneer, Johnston, IA
Julie Ritland , Plant Protection, DuPont Pioneer, Johnston, IA
Timothy M. Nowatzki , DuPont Pioneer, Johnston, IA
Tim Mabry , DuPont Pioneer, Ivesdale, IL
James Presnail , Evogene inc., Saint Louis, MO
Albert Lu , Plant Protection, DuPont Pioneer, Johnston, IA
RNA interference (RNAi) is a promising new technology for corn rootworm (CRW) control. We present the discovery of new gene targets DvSSJ1 and DvSSJ2, in CRW. DvSSJ1 and DvSSJ2 are orthologs of Drosophila genes snakeskin (Ssk) and mesh, respectively which encode membrane proteins associated with smooth septate junctions (SSJ) that are required for intestinal barrier function. Greenhouse and field experiments have demonstrated significant plant protection and insecticidal activity in transgenic events expressing DvSSJ1 dsRNA.
See more of: Ten-Minute Papers, P-IE Section: IPM - Field Crops 1
See more of: Ten Minute Paper (TMP) Oral
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