Variation of Culex quinquefasciatus cytochromes P450 (CYP6AA7, CYP4C52v1, CYP6BY3, CYP9J34, CYP9M10, CYP9J40, CYP9AL1, CYP6P14 and CYP9J45) genes sequence from many cities of Nuevo Leon (state), México

INTRODUCTION: The genome of Culex quinquefasciatus, a major mosquito/vector associated with disease transmission has been completed and annotated thousands of genes, within annotations have identified a large number of genes of the family of the P450 monooxygenase cytochromes (P450). METHODS: By RT-PCR were amplified nine cytochromes P450 (CYP6AA7, CYP4C52v1, CYP6BY3, CYP9J34, CYP9M10, CYP9J40, CYP9AL1, CYP6P14 and CYP9J45) transcripts from Culex quinquefasciatus, amplicons were cloned and nucleotide sequence was determined. RESULTS: The amplicons generated corresponded to the predicted size, non-other PCR products were visualized in electrophoretic agarose gel, thus we discard the possibility of alternative spliced mRNA species, the transcripts homology percentage varied differently among the different locations as well as to orthologous sequences previously published in the genome. DISCUSSION: A serial of re-arrays in the core of the sequences were visualized in Culex quinquefasciatus P450 monooxygenase cytochromes transcript. Constant exposure of insects to insecticides exerts selective pressure on organisms resulting in mutations, such changes are responsible for providing resistance as a means of survival, that to express higher levels of genes related to metabolism or detoxification or modifying the stereochemistry of target receptors to insecticides. Future physiological studies that give meaning to the mutations discovered open the panorama of pharmacogenomics in insects; and thus suggest new biological control mechanisms of Culex quinquefasciatus.